Microscale measurement of kinetic binding properties of monoclonal antibodies in solution using Gyrolab

The numbers of monoclonal antibodies approved for therapeutic use has increased rapidly over the last decade. As a consequence, precise and robust kinetic characterization techniques are crucial in order to select the best suitable candidates. A kinetic characterization method was developed in Gyrolab with automated sample transfers. The characterization was performed in solution in a mixing CD, containing an integrated nanoliter mixing chamber with affinity binding columns. Association rate constants were determined for four anti-TSH antibodies with values ranging from 3x10 5 M-1 s-1 to 10x10 5 M-1 s-1. The antibodies were ranked according to k ass. Reproducibility tests indicated that the method was robust and reproducible with CV-values around 10%.